DELFIA TRF Assays
DELFIA® (dissociation-enhanced lanthanide fluorescent immunoassay) TRF technology combines the benefits of time resolved fluorescence (TRF) with the use of lanthanide chelates for a highly sensitive, robust and reproducible assay format. With over 20 years of history, and thousands of publications, DELFIA is ideal for simple to complex matrices and can be used for both biochemical and cell-based assays.
The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. By combining the benefits of TRF with the use of lanthanide chelates, DELFIA offers multiple advantages over standard fluorometric detection:
- High sensitivity (femtogram level detection)
- Wide dynamic range (4-5 log)
- High S:B
- Large stokes shift
- Superior stability – (days)
- Multiplexing capability
From immunoassays, to binding and enzymatic studies, to cytotoxicity and cell proliferation assays, our DELFIA TRF assays are sure to provide you with unmatched sensitivity, time and time again.
For research use only. Not for use in diagnostic procedures.
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Target area
Explore cell-based and biochemical assays by target area of interest, including cell and gene therapy, GPCRs, targeted protein degradation, and more.
Explore cell-based and biochemical assays by target area of interest, including cell and gene therapy, GPCRs, targeted protein degradation, and more.
- Biologics screening and characterization: Explore assays for biologics screening, mechanism-of-action studies, and biomanufacturing.
- Biomarker detection: Accurate detection and quantitation of molecular targets across serum, plasma, culture supernatants, cellular lysates, and other sample types.
- Cell and gene therapy: Discover and advance cell and gene therapy research with bioanalytical immunoassays for CGT development.
- Cytokines: Access a comprehensive range of cytokine assay kits, including interleukins, interferons, chemokines, tumor necrosis factors, and lymphokines.
- Epigenetics: Leverage our epigenetics toolbox, designed to detect specific methylation and acetylation marks on histone peptide substrates.
- GPCRs: Measure cAMP levels and track downstream protein phosphorylation with functional GPCR assays.
- Protein kinases: Explore an extensive portfolio of protein phosphorylation assays, available in cell-based or biochemical no-wash formats.
- Protein-protein interactions: Utilize highly sensitive assays to study protein interactions involved in a range of biological processes.
- Targeted protein degradation: Monitor protein expression levels and characterize the mechanism of action using our total and phospho kits.
Therapeutic area
Explore cell-based and biochemical assays by your therapeutic area of research, including oncology, neuroscience, metabolic diseases, and more.
Explore cell-based and biochemical assays by your therapeutic area of research, including oncology, neuroscience, metabolic diseases, and more.
- Autoimmune diseases: Comprehensive reagents for autoimmune disease research, including rheumatoid arthritis, lupus, inflammatory bowel disease, multiple sclerosis, and more.
- Obesity, diabetes, and metabolic diseases: Solutions for drug discovery in obesity, diabetes, and metabolic diseases, featuring assays for biomarker detection, protein analysis, and GPCR research.
- Fibrosis: Ready-to-use assays for monitoring biomarkers, phosphoproteins, and transcription factors relevant to fibrotic disease research.
- Immuno-oncology: Assays for studying immunity and immune cells, covering protein, biomarker, and cytokine detection, as well as cell toxicity and proliferation.
- Oncology: A comprehensive oncology portfolio, including assays for cytokines, biomarkers, phospho-proteins, cell proliferation, cytotoxicity, and more.
- Neuroscience: A wide range of neuroscience assays to support research in neurodegenerative disorders such as Alzheimer’s, Parkinson’s, and Huntington's disease.
- Virology: Assay technologies for viral studies, enabling assessment of viral life cycles and antiviral immune responses with streamlined workflows.
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1 - 25 of 57 Products and Services
DELFIA® time-resolved fluorescence (TRF) cell proliferation kit for measuring cytotoxicity or proliferation in mammalian cells.
Europium-labeled anti-mouse IgG antibody, for capturing antibodies in DELFIA® TRF assays.
Europium-labeled anti-HA antibody for binding HA-tagged proteins in DELFIA® time-resolved fluorescence (TRF) assays. The anti-HA antibody is a purified IgG2b subclass of mouse monoclonal antibody. It recognizes the epitope sequence (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein.
Europium-labeled anti-HRP IgG antibody, for use in DELFIA® TRF assays.
Europium-labeled anti-goat IgG antibody, for use in DELFIA® TRF assays
DELFIA® Europium labeling kit for labeling your own biomolecules for use in DELFIA time-resolved fluorescence assays. The ITC chelate reacts with free amine groups in the target biomolecule.
Europium-labeled anti-c-myc antibody, for capturing c-myc-tagged proteins in LANCE® TR-FRET assays.
Europium-labeled anti-phosphotyrosine (PY20) antibody, for use in DELFIA time-resolved fluorescence (TRF) kinase assays, phosphoprotein detection, and phosphoprotein binding assays.
Europium-labeled anti-rabbit IgG antibody, for use in DELFIA® TRF assays
Europium-labeled anti-phosphothreonine antibody, for use in DELFIA® time-resolved fluorescence (TRF) kinase assays.
DELFIA® Europium-labeled anti-human IgG, for use in DELFIA time-resolved fluorescence (TRF) immunoassays.
Europium-labeled anti-c-myc antibody, for capturing c-myc-tagged proteins in DELFIA® TRF assays.
DELFIA® TRF (time-resolved fluorescence) cytotoxicity kit for measuring chemical cytotoxicity, cell-mediated cytotoxicity, and antibody-dependent cell cytotoxicity (ADCC). This kit has the same assay principle as a chromium release assay, with target cells loaded with a BATDA ligand instead of 51Cr. There is no requirement for specially-engineered cells.
Wash buffer for DELFIA® time-resolved fluorescence (TRF) ligand binding assays.
DELFIA Enhancer solution for forming fluorescent Terbium and Dysprosium chelates in DELFIA time-resolved fluorescence (TRF) assays.
Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The DTA-activated chelate reacts with free amino groups, sulfhydryl groups, and hydroxyl groups. Because of its high reactivity, we usually do not recommend DTA as it may “inactivate” the reagent you are labeling.
Terbium labeling chelate for creating your own Terbium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The ITC-activated chelate reacts primarily with free amino groups, such as lysines.
Terbium labeling chelate for creating your own Terbium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The ITC-activated chelate reacts with free amino groups, such as lysines. The DTPA chelate is more-stable than the N1 chelate.
Concentrated washing solution for DELFIA® time-resolved fluorescence (TRF) assays.
DELFIA® Europium labeling chelate and Europium standard for labeling your own biomolecules for use in DELFIA time-resolved fluorescence assays. The ITC chelate reacts with free amine groups in the target biomolecule.
Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The amino-activated chelate reacts with free carboxyl groups. The DTPA chelate is more-stable than the N1 chelate.
100 nmol/L Fluorescein standard solution, 50 mL Shipped on blue ice.
DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.
DELFIA® Europium solution for use in time-resolved fluorescence (TRF) cytotoxicity assays.
DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.
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1 - 15 of 15 Resources
Applicability of AlphaLISA technology to a wide spectrum of complex biological samples
DELFIA Assays: Flexible and Sensitive Tools for Monoclonal Antibody Development
Developing a LANCE TR-FRET assay for screening PD-1 and PD-L1 binding inhibitors.
Guidelines for optimization of a cell-labelling method for DELFIA-TRF cytotoxicity assays.
Optimization and miniaturization of DELFIA TRF assays converted from ELISA.
Qualification of a DELFIA Assay for the Detection of Anti-mouse IgG Antibodies in Human Serum
Simultaneous measurement of chemokine/cytokine release and antibody-dependent cellular cytotoxicity upon binding with rituximab
Utilization of AlphaLISA technology to accurately detect asthma biomarkers in human serum
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