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Application Note

Evaluation of protein extraction efficiency from hard tissues using the Omni Bead Ruptor 12 bead mill homogenizer

Protein extraction efficiency from hard tissues

The efficiency of tissue homogenization for protein extraction is an important consideration upstream of many basic and pre-clinical research applications, including proteomic studies. Bead mill homogenization, such as with the Omni Bead Ruptor 12 homogenizer, facilitates uniform processing of multiple samples, achieving full homogenization in just 60 seconds to yield a consistent protein recovery of 7.2 ± 0.71 mg from ~50 mg of rat heart tissue, with less than 10% deviation as observed by SDS-PAGE. Labs can leverage the bead mill as an effective automation-enabled solution for high-throughput protein extraction from hard, dense tissues such as the heart in quantitative proteomic analyses compared to more manual methods, making it ideal for tissue homogenization for protein extraction.

For research use only. Not for use in diagnostic procedures.

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Evaluation of protein extraction efficiency from hard tissues using the Omni Bead Ruptor 12 bead mill homogenizer

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