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Technical Note

Guidelines for optimizing tyrosine phosphorylation detection: Effective use of pervanadate in kinase small molecule inhibitors studies

Tyrosine phosphorylation is a critical regulatory mechanism in cell signaling, impacting cancer, autoimmune disorders, and targeted drug discovery. However, detecting tyrosine phosphorylation can be challenging due to its transient nature. This technical note provides essential guidelines for using pervanadate, a potent phosphatase inhibitor, to enhance phosphorylation detection and improve kinase inhibitor studies.

Learn how to:

  • Prepare and handle pervanadate safely
  • Optimize experimental conditions for accurate detection
  • Reduce cytotoxic effects while maintaining cell viability

Discover the technical note demonstrating the effective use of pervanadate with HTRF detection kits.

For research use only. Not for use in diagnostic procedures.

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Guidelines for optimizing tyrosine phosphorylation detection: Effective use of pervanadate in kinase small molecule inhibitors studies