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Application Note

Nucleic acid recovery from multiple tissues with scalable throughput utilizing the KingFisher Flex and Omni Bead Ruptor Elite bead mill homogenizer

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In this study, the Omni Bead Ruptor Elite homogenizer was integrated into a semi-automated workflow for high-throughput nucleic acid extraction from various mouse tissues, including liver, kidney, heart, skin, skeletal muscle, and lungs. An efficient protocol using off-the-shelf chemistry was developed and our results demonstrate its robust scalability, where a processing range from 10 – 100 mg showed proportional increases in nucleic acid yield for most tissue types with this bead mill and KingFisher™ Flex system combination. While liver and kidney samples showed some saturation effects at higher masses, the homogenization parameters and the extraction protocol employed, generally produced nucleic acids that could be reliably feed into common downstream applications such as PCR and sequencing. The workflow, therefore, offers a reliable solution and path to automation for researchers working with multiple types of complex tissue and looking to improve laboratory efficiency.

For research use only. Not for use in diagnostic procedures.

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Nucleic acid recovery from multiple tissues with scalable throughput utilizing the KingFisher Flex and Omni Bead Ruptor Elite bead mill homogenizer

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