RNA extraction from murine tissues: Balancing preservation and extraction parameters for cryogen-free processing

Efficient RNA extraction is important to the success of downstream assays, including transcriptomic analysis. RNA can be fragile and be prone to degradation, so labs consider a range of factors, including sample storage conditions, sample homogenization, and extraction methods that could help obtain robust yields and integrity scores while permitting a practical solution to streamline or standardize protocols for diverse tissue samples.

In this application note, review performance data from mouse liver, skeletal muscle, and skin:

• Comparative data across three sample acquisition/storage parameters: fresh, frozen, and frozen in RNAlater™
• Resulting RNA yield, RIN data, and statistical analysis from tissue extraction on ice or at room temperature
• Optimized homogenization parameters for each tissue type using bead mill homogenization

For research use only. Not for use in diagnostic procedures.