Quantification inflammatory cytokines skin serum OmniBRElite

Cytokine release syndrome (CRS) – a systemic inflammatory condition triggered by the activation of immune cells such as NK cells, macrophages, and dendritic cells – is characterized by high levels of inflammatory cytokines and can be triggered by infections causing sepsis or by treatments such as CAR-T cell therapy, among other factors. The resulting feedback loop of intensified inflammation, generally, could also lead to severe tissue damage. Therefore, the ability to consistently quantify local immune mediators is important for research labs looking to understand CRS and how to mitigate its detrimental effect on the body.

In this application note

• Review comparative performance data between a probe sonicator and a bead mill homogenizer on protein analyte levels detected from skin samples
• Access data from serum and skin samples for 13 cytokines measured (13-plex) using the LEGENDplex™ Mouse Cytokine Release Syndrome Panels from an LPS-induced murine model

Results

Cytokine levels from serum and skin:

• The levels of all 13 cytokines measured from serum using the LEGENDplex Cytokine Release Syndrome panel were higher following LPS stimulation compared to PBS control (Figures 2-5).
• The levels of all 13 cytokines measured in the LPS group from skin samples that were lysed using the Omni
  Bead Ruptor Elite bead mill homogenizer (LPS BRE) (Figures 6-9) were:
  • Higher compared to skin samples in LPS group prepared using the sonicator method (LPS Sonic).
  • Higher compared to PBS control skin samples prepared using the sonicator (PBS Sonic) and bead mill
    homogenizer (PBS BRE) methods.
• Cytokine levels measured in skin samples prepared using the sonicator method (Sonic) were remarkably lower compared to those prepared using the bead mill homogenizer method (BRE) (Figures 6-9).

For research use only. Not for use in diagnostic procedures.