The AlphaLISA™ SureFire® Ultra™ total RIPK1 assay is a sandwich immunoassay for quantitative detection of RIPK1 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.
Feature | Specification |
---|---|
Application | Cell Signaling |
Sample Volume | 30 µL |
The AlphaLISA™ SureFire® Ultra™ total RIPK1 assay is a sandwich immunoassay for quantitative detection of RIPK1 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.
The AlphaLISA™ SureFire® Ultra™ total RIPK1 assay is a sandwich immunoassay for quantitative detection of total RIPK1 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology.
Formats:
In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.
AlphaLISA SureFire Ultra kits are compatible with:
Alpha SureFire kits can be used for:
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Modality |
Alpha
|
Lysis Buffer Compatibility |
Lysis Buffer
|
Molecular Modification |
Total
|
Product Group |
Kit
|
Sample Volume |
30 µL
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
RIPK1
|
Target Class |
Phosphoproteins
|
Target Species |
Human
|
Technology |
Alpha
|
Therapeutic Area |
Inflammation
Neuroinflammation
Oncology
|
Unit Size |
100 assay points
|
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