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AlphaLISA SureFire Ultra Human & Mouse Total STAT3 Detection Kit, 500 Assay Points
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AlphaLISA SureFire Ultra Human & Mouse Total STAT3 Detection Kit, 500 Assay Points
AlphaLISA Surefire Ultra Total Protein
The AlphaLISA™ SureFire® Ultra™ Total STAT3 assay is a sandwich immunoassay for quantitative detection of STAT3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Sample Volume | 10 µL |
The AlphaLISA™ SureFire® Ultra™ Total STAT3 assay is a sandwich immunoassay for quantitative detection of STAT3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Product Variants
Unit Size: 500 assay points
Part #:
ALSU-TST3-A500
List Price
USD 2,392.92
Your online price:
Unit Size: 10,000 assay points
Part #:
ALSU-TST3-A10K
List Price
USD 14,400.00
Your online price:
Unit Size: 50,000 assay points
Part #:
ALSU-TST3-A50K
List Price
USD 46,000.00
Your online price:
Unit Size: 100 assay points
Part #:
ALSU-TST3-A-HV
List Price
USD 708.33
Your online price:
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Human & Mouse Total STAT3 Detection Kit, 500 Assay Points
AlphaLISA Surefire Ultra Total Protein
AlphaLISA SureFire Ultra Human & Mouse Total STAT3 Detection Kit, 500 Assay Points
Product information
Overview
The AlphaLISA™ SureFire® Ultra™ Total STAT3 assay is a sandwich immunoassay for quantitative detection of STAT3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
In the AlphaLISA™ SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.
AlphaLISA™ SureFire® Ultra™ kits are compatible with:
- Cell and tissue lysates
- Antibody modulators
- Biotherapeutic antibodies
Alpha SureFire® kits can be used for:
- Cellular kinase assays
- Receptor activation studies
- Screening
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Cellular or Signaling Pathway |
JAK/STAT
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Total
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
STAT3
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
Mouse
|
| Technology |
Alpha
|
| Unit Size |
500 assay points
|
Video gallery
AlphaLISA SureFire Ultra Human & Mouse Total STAT3 Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human & Mouse Total STAT3 Detection Kit, 500 Assay Points
Resources
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Guide
AlphaLISA SureFire Ultra assay optimization
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
Guide
AlphaLISA SureFire Ultra: the ultimate guide for successful experiments
The definitive guide for setting up a successful AlphaLISA SureFire Ultra assay
Several biological processes are regulated by...
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USD 708.33 - USD 46,000.00
USD 708.33 - USD 46,000.00
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