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HTRF Human Phospho-α-Synuclein (Ser129) Aggregation Kit, 500 Assay Points

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HTRF Human Phospho-α-Synuclein (Ser129) Aggregation Kit, 500 Assay Points
Shipping box for Revvity reagent kits
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Shipping box for Revvity reagent kits
Shipping box for Revvity reagent kits
Shipping box for Revvity reagent kits
Shipping box for Revvity reagent kits
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This HTRF enables the cell-based quantitative detection of Alpha-Synuclein when phosphorylated at Ser129 and aggregated.

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Feature Specification
Application Cell Signaling
Sample Volume 16 µL
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This HTRF enables the cell-based quantitative detection of Alpha-Synuclein when phosphorylated at Ser129 and aggregated.

Click to copy promo code to clipboard.
March lab savings - SAVE 10%. Use promo code below.
MARCH10
Terms and conditions apply.
View product information
Product Variants
Unit Size: 500 assay points
Part #:
64SYN29AGPEG
List Price
USD 2,271.53
Your online price:
Unit Size: 10,000 assay points
Part #:
64SYN29AGPEH
List Price
USD 13,214.42
Your online price:
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
Total list price:
USD 2,271.53
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Shipping box for Revvity reagent kits
Shipping box for Revvity reagent kits
Video Play Button
HTRF Human Phospho-α-Synuclein (Ser129) Aggregation Kit, 500 Assay Points
Shipping box for Revvity reagent kits
Video Play Button
Shipping box for Revvity reagent kits
Shipping box for Revvity reagent kits
HTRF Human Phospho-α-Synuclein (Ser129) Aggregation Kit, 500 Assay Points
Shipping box for Revvity reagent kits
Shipping box for Revvity reagent kits
Video Play Button

Product information

Overview

Monitoring the phosphorylation and aggregation states of alpha-synuclein is of great interest for studying the pathogenesis of synucleinopathies, a group of neurodegenerative diseases that includes Parkinson's disease (PD), dementia with Lewy bodies (DLB), diffuse Lewy body disease (DLBD), and multiple system atrophy (MSA). The kit provides a solution to rapidly detect and quantify protein aggregates of human alpha-synuclein in cell culture or brain tissue extracts.

Specifications

Application
Cell Signaling
Brand
HTRF
Detection Modality
HTRF
Lysis Buffer Compatibility
Lysis Buffer 5
Molecular Modification
Phosphorylation
Product Group
Kit
Sample Volume
16 µL
Shipping Conditions
Shipped in Dry Ice
Target Class
Phosphoproteins
Target Species
Human
Technology
TR-FRET
Unit Size
500 assay points

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HTRF Human Phospho-α-Synuclein (Ser129) Aggregation Kit, 500 Assay Points
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HTRF Human Phospho-α-Synuclein (Ser129) Aggregation Kit, 500 Assay Points
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How it works

Principle of the HTRF (h) Aggregated Phospho-A-Synuclein Ser129 assay

Alpha-Synuclein aggregates are measured using a sandwich immunoassay involving an anti-a Synuclein monoclonal antibody specific for phospho-Ser129 Alpha-Synuclein aggregates labelled with terbium-Cryptate or d2, ensuring assay quality, reproducibility, and signal quality. The specific HTRF signal generated is proportional to the alpha-Synuclein aggregates.

assay-principle-how-it-works-alpha-synuclein-aggr-phosphos129-64syn29agpeg-64syn29agpeh-64syn29agpey.svg
Protocol of the HTRF Human Aggregated Phospho-A-Synuclein Ser129 assay

The sample or brain extracts are transferred to the assay plate for the detection of Alpha-Synuclein aggregates. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.

assay-protocol-how-it-works-alpha-synuclein-aggr-phosphos129-64syn29agpeg-64syn29agpeh-64syn29agpey.svg

 

Assay validation

Validation of Aggregated Phospho-A-Synuclein Ser129 detection

A sample generated with aggregated phospho-Alpha-Synuclein (S129) was purified by exclusion phase (G25), resulting in 3 peaks corresponding respectively to oligomers, dimers, and monomers of phospho-Alpha-Synuclein.

Each sample resulting from this purification step was serial-diluted 14 times and plated at 16 µL/well in a low volume white microplate.

2µL of the HTRF d2 detection reagent and 2µL of the HTRF Eu-Tb detection reagent were added, and the signal was recorded after an overnight incubation at RT.

The results show significant detection of the oligomer aggregates only. Closer examinations of the dimer aggregates' detection showed a lower but still detectable signal. Monomers were not detected, demonstrating the specificity of the assay for aggregates only.

assay-validation-alpha-synuclein-aggr-phosphos129-64syn29agpeg-64syn29agpeh-64syn29agpey-01.svg
assay-validation-alpha-synuclein-aggr-phosphos129-64syn29agpeg-64syn29agpeh-64syn29agpey-02.svg

 

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