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HTRF SARS-Cov2 Spike S1 protein Detection Kit, 10,000 Assay Points

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HTRF SARS-Cov2 Spike S1 protein Detection Kit, 10,000 Assay Points
Shipping box for Revvity reagent kits
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Shipping box for Revvity reagent kits
Shipping box for Revvity reagent kits
Shipping box for Revvity reagent kits
Shipping box for Revvity reagent kits
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This HTRF kit is designed to detect SARS-CoV-2 Spike S1 proteins in cell lysates or cell supernatant, as a marker of viral infection.

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MARCH10
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Feature Specification
Application Protein Quantification
Dynamic Range 66 - 16,000 pg/mL
Limit of Detection 15 pg/mL
Sample Volume 16 µL
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This HTRF kit is designed to detect SARS-CoV-2 Spike S1 proteins in cell lysates or cell supernatant, as a marker of viral infection.

Click to copy promo code to clipboard.
March lab savings - SAVE 10%. Use promo code below.
MARCH10
Terms and conditions apply.
View product information
Product Variants
Unit Size: 500 assay points
Part #:
63ADK114PEG
List Price
USD 1,881.00
Your online price:
Unit Size: 10,000 assay points
Part #:
63ADK114PEH
List Price
USD 25,143.46
Your online price:
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
Total list price:
USD 25,143.46
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Shipping box for Revvity reagent kits
Shipping box for Revvity reagent kits
Video Play Button
HTRF SARS-Cov2 Spike S1 protein Detection Kit, 10,000 Assay Points
Shipping box for Revvity reagent kits
Video Play Button
Shipping box for Revvity reagent kits
Shipping box for Revvity reagent kits
HTRF SARS-Cov2 Spike S1 protein Detection Kit, 10,000 Assay Points
Shipping box for Revvity reagent kits
Shipping box for Revvity reagent kits
Video Play Button

Product information

Overview

The SARS-CoV-2 Spike S1 protein is one of two part of the virus Spike protein (S). The S1 subunit is responsible for the the virus interaction with host cells' transmembrane Angiotensin-converting enzyme 2 receptors (ACE2), while the S2 subunit is capable of conformational changes that allow the fusion of the viral particle envelope and host cell membrane upon S1/ACE2 binding. As such, the S1 protein is closely investigated for its role in viral infection and is reliable marker of infection as well as a potential vaccine immunogen target.

Specifications

Application
Protein Quantification
Brand
HTRF
Detection Modality
HTRF
Dynamic Range
66 - 16,000 pg/mL
Limit of Detection
15 pg/mL
Product Group
Kit
Sample Volume
16 µL
Shipping Conditions
Shipped in Dry Ice
Target Class
Viral Particles
Technology
TR-FRET
Unit Size
10,000 assay points

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HTRF SARS-Cov2 Spike S1 protein Detection Kit, 10,000 Assay Points
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HTRF SARS-Cov2 Spike S1 protein Detection Kit, 10,000 Assay Points
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How it works

SARS-CoV-2 particles are detected in cell lysates using a sandwich immunoassay involving an anti-S1 monoclonal antibody, labelled with terbium-Cryptate or d2, ensuring assay quality, reproducibility, and signal quality. The specific HTRF signal generated is proportional to the amount of S1 proteins.

1biomarkers-how-it-works-assay-principle-spike-s1-protein-kit-63adk114peg-63adk114peh.svg

 

The assay protocol, using a 384-well small volume plate or a low volume 96 well plate (20 µL final), is described on the right. 16 µL of cell lysates or control are dispensed directly into the detection plate for detection by HTRF reagents. The antibodies labelled with HTRF donor and acceptor can be pre-mixed and added in a single dispensing step to further streamline the assay procedure (4 µL), or separately (2 µL).

2biomarkers-how-it-works-assay-protocol-spike-s1-protein-kit-63adk114peg-63adk114peh.svg

 

Assay details

Detection of recombinant SARS-CoV-2 S1 protein

16 µL of recombinant protein in lysis buffer #5 were dispensed into a low volume white microplate before the addition of 4 µL of the premixed HTRF anti SARS-CoV2 capsid detection reagents. The HTRF signal was recorded after ON incubation.

 

 

3assay-validation-spike-s1-protein-kit-1.svg

 

standard curve Spike S1 kit
Sample size 16 µL
Final assay volume 20 µL
Time to result ON at RT
Detection limit 15 pg/mL
Dynamic range 66 to 16,000 pg/mL
Sample compatibility Cell-based or cell supernatant

 

 

4assay-validation-spike-s1-protein-kit-std.svg

 

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