The LANCE® Ultra Human MMP-10 Detection Kit is designed for detection and quantitation of human MMP-10 in cell culture media and cell supernatants using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Feature | Specification |
---|---|
Application | Protein Quantification |
Dynamic Range | 13.2- 100,000 pg/mL |
Limit of Detection | 13.2 pg/mL |
Limit of Quantification | 66.1 pg/mL |
Sample Volume | 15 µL |
The LANCE® Ultra Human MMP-10 Detection Kit is designed for detection and quantitation of human MMP-10 in cell culture media and cell supernatants using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
The LANCE Ultra Human MMP-10 Detection Kit is designed for detection and quantitation of human fibrinogen in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Features:
LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Matrix Metalloproteinase 10 (MMP10) is also known as stromelysin-2 or transin-2. MMPs are involved in the breakdown of the extracellular matrix in normal physiological processes. MMP10 is secreted in a “proMMP10” form with is cleaved by other extracellular proteinases to become active. MMP10 has been linked to cancer stem cell vitality and metastasis and has been tabbed as a potential marker in oral cancer.
Application |
Protein Quantification
|
---|---|
Automation Compatible |
Yes
|
Brand |
LANCE Ultra
|
Detection Modality |
LANCE
|
Dynamic Range |
13.2- 100,000 pg/mL
|
Limit of Detection |
13.2 pg/mL
|
Limit of Quantification |
66.1 pg/mL
|
Product Group |
Kit
|
Sample Volume |
15 µL
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
MMP10
|
Target Class |
Biomarkers
|
Target Species |
Mouse
|
Technology |
TR-FRET
|
Therapeutic Area |
Oncology
|
Unit Size |
500 Assay Points
|
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LANCE™ Ultra TR-FRET is a no-wash proximity assay technology that combines the benefits of time resolution (TR) with fluorescence...
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