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NEXTFLEX Universal Blockers

The NEXTFLEX® Universal Blockers are short DNA oligos block non-specific hybridization between adapter sequences, enhancing specificity and reducing sequencing costs. They are compatible with all NEXTFLEX barcodes and all other ligation and tagmentation based workflows for Illumina® and Element® sequencing platforms.

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MARCH10
Feature Specification
Automation Compatible No
Product Group Targeted Sequencing

The NEXTFLEX® Universal Blockers are short DNA oligos block non-specific hybridization between adapter sequences, enhancing specificity and reducing sequencing costs. They are compatible with all NEXTFLEX barcodes and all other ligation and tagmentation based workflows for Illumina® and Element® sequencing platforms.

Click to copy promo code to clipboard.
March lab savings - SAVE 10%. Use promo code below.
MARCH10
Product Variants
Unit Size: 8 rxns
Part #:
NOVA-5143231
List Price
USD 113.41
Your online price:
Unit Size: 48 rxns
Part #:
NOVA-5143232
List Price
USD 237.54
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Unit Size: 96 rxns
Part #:
NOVA-5143233
List Price
USD 373.42
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For research use only. Not for use in diagnostic procedures.
Total list price:
USD 373.42
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Overview

Simplify and reduce the cost of hybridization-based target capture with the NEXTFLEX universal blockers for NGS
  • Effective in blocking without being affected by index length or the presence of a unique molecular index (UMI)
  • Compatible with all NEXTFLEX barcodes and all other ligation and tagmentation based workflows for Illumina and Element sequencing platforms
  • Simplifies and reduces the cost of target capture without sacrificing performance

Additional product information

Improve the Number of On-Target Reads

During target enrichment, adapters can interact with the complementary adapter sequence strand of another library molecule. The NEXTFLEX Universal Blockers are designed to prevent this concatemerization of library molecules. By blocking this complementary adapter sequence interaction, the number of on-target reads improves dramatically and the depth of enrichment increases.

One Set of Blockers for All Applications

The NEXTFLEX Universal Blockers are designed for use with indexed adapters for Illumina sequencing platforms. They are effective in blocking single-indexed or dual-indexed libraries, without being affected by index length or the presence of a unique molecular index (UMI).

nextflex universal blockers figure 1

Figure 1. NEXTFLEX Universal Blockers (UB) work from 0-19 bp unblocked regions (barcodes and/or UMIs). Competitor A1- and A-3 are two separate products from Competitor A.

nextflex universal blockers figure 2

Figure 2. NEXTFLEX Universal Blockers can block Illumina TruSeq™ or Nextera™ -style libraries. Competitor A1- and A-2 are two separate products from Competitor A.

The NEXTFLEX Universal Blockers are compatible with ligation-based workflows, such as NEXTFLEX or Illumina TruSeq library prep kits, and with any libraries prepared with NEXTFLEX Dual-Index Barcodes or NEXTFLEX Unique Dual Index Barcodes.

They are also compatible with tagmentation-based workflows, such as Illumina Nextera library prep kits.

Specifications

Automation Compatible
No
Product Group
Targeted Sequencing
Shipping Conditions
Shipped in Dry Ice
Unit Size
96 rxns

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Simply & reduce the cost of hybridization-based target capture with universal blockers

This flyer describes the benefits of the NEXTFLEX® Universal Blockers

SDS, COAs, Manuals and more

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Adapters (10NT index)

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Part number:NOVA-534100
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Revvity kit box for reagents and consumables
Part number:NOVA-534101
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Revvity kit box for reagents and consumables
Part number:NOVA-534102
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Revvity kit box for reagents and consumables
Part number:NOVA-534103
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Revvity kit box for reagents and consumables
Part number:NOVA-534104
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Adapters (8NT index)

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Part number:NOVA-514150
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Part number:NOVA-514150-EVAL16
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Revvity kit box for reagents and consumables
Part number:NOVA-514150-EVAL48
List price:USD 489.60/each
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Cell-Free DNA-seq Library Prep

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Part number:NOVA-5150-01
List price:USD 192.00/each
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Revvity kit box for reagents and consumables
Part number:NOVA-5150-02
List price:USD 1,152.00/each
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DNA-seq Library Prep (Enzymatic Fragmentation)

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Part number:NOVA-5149-21
List price:USD 192.00/each
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Part number:NOVA-5149-22
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Part number:NOVA-5149-23
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DNA-seq Library Prep (Mechanical Fragmentation)

Revvity kit box for reagents and consumables
Part number:NOVA-5188-01
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Revvity kit box for reagents and consumables
Part number:NOVA-5188-02
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