PhenoVue Fluor 405 – Phalloidin

Phalloidin is a toxin isolated from the fungus Amanita phalloides. It is a bicyclic peptide that specifically binds and stabilizes actin filaments.

Fluorescent Phalloidin derivatives have been extensively used for monitoring actin expression and dynamics of the cytoskeleton network. The study of actin cytoskeleton regulation is of interest in the context of several pathophysiological processes such as cancer and neurodegenerative diseases as well as fibrosis and vascular diseases.

PhenoVue Fluor 405 – Phalloidin can be used to visualize actin filaments in indirect immunofluorescence, immunohistochemistry as well as high-content analysis and screening applications. ​​Its photophysical property enables extended multiplexing capability by adding a fifth color while avoiding spectral overlap and providing high brightness as well as photostability.

Quantity per pack: 300 units (10 nmoles; 11 µg)

Suggested working concentration: 165 nM (0.17 µg/mL)

Equivalent number of microplates:

• 2 - 6 x 96-well microplates
• 2 - 6 x 384-well microplates
• 3 - 10 x 1536-well microplates