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Article
Busting high-content imaging myths
High-content imaging has become a cornerstone of life science research, providing valuable insights into cellular function and drug responses. However, as with any advanced technology, misconceptions and myths can emerge. In this article, we’ll debunk some of the most common myths surrounding high-content imaging, shedding light on its true strengths and limitations.
Literature - Publication Review
Developing a phenotypic in vitro model for progression of liver steatosis
A recent study developed a model of hepatic steatosis in functional human induced pluripotent stem cell-derived hepatocytes (hiPSC-Hep).
Case Study
A workflow to characterize and benchmark human induced pluripotent stem cells
Case study describing a high-content imaging workflow to characterize and benchmark human induced pluripotent stem cells
Literature - Publication Review
Artemisinins target GABAA receptor signaling and impair α cell identity
Artemisinins Target GABA Receptor Signaling and Impair α Cell Identity encouraging α cells to convert into insulin-producing β-like cells.
Case Study
High-content analysis of drug-induced oligodendrocyte differentiation promoting remyelination in multiple sclerosis
Case study describing High-Content Analysis of Drug-Induced Oligodendrocyte Differentiation Promoting Remyelination in Multiple Sclerosis
Application Note
A multiparametric live-cell cytotoxicity analysis using the Operetta High-content Analysis System
An in vitro cytotoxicity approach using HepG2 cells, which are used as an effective indicator of the human hepatotoxicity potential of test compounds.
Application Note
Analyzing ERK signal transduction in live cells using a FRET-based biosensor
Application note describing analysis of ERK signal transduction in live cells using a FRET-based biosensor with the Operetta CLS high-content analysis system.
Case Study
B cell selection and therapeutic antibody characterization using the Operetta high content imaging system
This case study illustrates how Revvity’s high content imaging solutions are enabling therapeutic antibody development.
Technical Note
3D volumetric and zonal analysis of solid spheroids
Technical note describing how to image and analyze solid spheroids in 3D.
Technical Note
Clearing strategies for 3D spheroids
In this technical note, we compare optical clearing strategies for 3D spheroids and demonstrate how to increase imaging depth in 3D spheroids by a factor of 4.
Application Note
Kinetic analysis of calcium flux activity in human iPSC-derived neurons using the Opera Phenix Plus system
Application note for fast kinetic imaging using HCS to visualize and evaluate spontaneous calcium flux activity in single human iPSC-derived neurons
Technical Note
Opera Phenix High-Content Screening System: Improved 3D imaging
Technical Note describing how imaging of 3D cell models is improved using the Opera Phenix High-Content Screening System
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