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Technical Note
Guidelines for cell culture and lysis in different formats prior to HTRF detection
This Technical Note will provide you with guidelines on adapting the standard 96-well plate protocol for seeding and lysing cells to different culture formats such as T75 flasks, or 6-well, 12-well, and 24-well plates.
Technical Note
The AlphaLISA™ Approach to TNFR1 Measurement: Insights from Human Urine Samples
Detailed in this technical document is the method for assessing TNFR1 concentrations in human urine, showcasing a comparative analysis between samples from healthy individuals and those with type 2 diabetes.
Technical Note
The Advantages of AlphaLISA™ Technology: A Study with Human TNFR1
This technical note compares ELISA kits from two suppliers to the Human TNFR1 AlphaLISA™ Detection Kit to demonstrate the ease of use and benefits of Alpha technology.
Technical Note
AlphaLISA SET7/9 Histone H3-lysine N-methyltransferase assay.
this AlphaLISA immunoassay detects the mono-methylation of a biotinylated Histone H3 (1-21) peptide at lysine 4.
Technical Note
AlphaLISA SIRT1 p53 lysine 382 deacetylase assay
This AlphaLISA immunodetection assay measures the deacetylation of a biotinylated p53 (368-393) peptide acetylated at lysine 382.
Guide
ELISA to AlphaLISA: Immunoassay conversion made easy
Discover streamlined immunoassays with AlphaLISA™. Convert ELISA to AlphaLISA for precision, proven technology, and versatile measurements.
Guide
AlphaPlex assay development guide
This guide equips Alpha users with the knowledge and tools to harness the full potential of multiplexing. Download to learn more!
Guide
Start-up guide: Alpha technology/protein: protein interactions
This quick start guide will help you set-up no-wash AlphaLISA assays for measuring protein:protein interactions.
Technical Note
AlphaLISA JMJD3 Histone H3-Lysine 27 demethylase assay
This AlphaLISA immunodetection assay measures the demethylation of a biotinylated Histone H3 (21-44) peptide tri-methylated at lysine 27.
Technical Note
Exposing and detecting membrane and intracellular proteins: AlphaLISA simplifies the detection of HO-1
This technical document reveals the application of AlphaLISA technology in measuring HO-1 concentrations within HeLa cell extracts.
Technical Note
Development of an AlphaLISA MEK1 kinase assay using full-length ERK2 substrate
This technical note illustrates one example of how these toolbox beads may be used to efficiently develop an assay for assessing MEK1 phosphorylation of a native ERK2 substrate.
Guide
AlphaLISA assay development guide
A few simple steps are needed to develop an AlphaLISA™ analyte assay. This protocol is the starting point for the development of all AlphaLISA assays.
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