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Application Note
HMGB1, IL-1β and IL-18 assays used together to discriminate pyroptosis and necroptosis
This application note shows that the release of HMGB1 induced in the context of pyroptosis and necroptosis can be easily monitored with the HTRF® HMGB1 kit.
Application Note
Download your application note on fibrosis characterization
This note describes deep investigations of the molecular mechanisms involved in tissue fibrosis, and occurring in the major effector cell types.
Application Note
Download your application note on nuclear receptor ligand identification with HTRF
This application note illustrates the use of HTRF PPI reagents to easily build robust and convenient biochemical NR/ CoA recruitment assays.
Application Note
Best practices for analyzing tumor xenografts
This technical note provides procedures and guidelines for performing HTRF phospho-protein assays on tumor xenografts, with a particular focus on the preparation of tumor lysates and working samples.
Application Note
Download your protocol on CD4 T cells differentiation into Th17 cells
In this application note is reported a protocol for the isolation of mouse naïve CD4+ T-cells and their differentiation into T-Reg and Th-17 cells.
Application Note
Download your application note on nuclear receptor ligand identification with HTRF
This application note illustrates the use of HTRF PPI reagents to easily build robust and convenient biochemical NR/CoA recruitment assays.
Application Note
A guideline for HTRF cell-based phospho-protein data normalization
This application note illustrates how to calculate a normalization value and interpret the results correctly when using htrf phosphospecific and total protein assays.
Application Note
HTRF probes all types of protein-protein interaction
HTRF probes all types of protein-protein interaction, from small to very large complexes
Application Note
Kinetic Binding of Kinase inhibitors and determination of Kon, Koff rates
This application note presents the use of fluorescent kinase inhibitors to determine the kinetic binding properties of inhibitors of interest and demonstrates the pharmacological relevance of the kinase binding platform for both equilibrium-and kinetic profiling.
Application Note
Characterizing pharmacological compounds with complementary Upstream and Downstream GPCR readouts
This application note presents and compares the pharmacological characterization of a panel of reference compounds (full agonists, partial agonists, antagonists) in δ-opioid and D2 receptor models with two different Gi-coupled receptor activity monitoring assays: the HTRF® GTP Gi binding assay and the HTRF cAMP Gi assay.
Application Note
Research on neutralizing antibodies in autoimmune diseases
The data presented here demonstrates that HTRF biochemical and cellular assays offer a comprehensive and easy-to-implement platform for the identification of potent biotherapeutics to address autoimmune pathologies.
Application Note
Neuroinflammation study using TREM2 and SYK signaling pathways in human iPSC-derived microglia-like cells
This application note demonstrates the power of HTRF for TREM2 and SYK signaling in human iPSC-Derived macrophages studies.
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