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Technical Note
Optimize your HTRF cell signaling assays for tissues
This technical note gives precise guidelines for tissue lysate preparation, assay optimization, data analysis, and then interpretation, guaranteeing you will get accurate and interpretable results.
Technical Note
Guidelines from PBMC isolation to cytokine assay optimization
This tech note will provide you with a complete step by step methodology to purify PBMCs and use them in cytokine quantification assays with HTRF
Technical Note
Package insert for the T-SPOT.TB test
The T-SPOT.TB test is an in vitro diagnostic test for the detection of effector T cells that respond to stimulation by Mycobacterium tuberculosis antigens and is intended for use as an aid in the diagnosis of tuberculosis (TB) infection. The T-SPOT.TB test is a simplified enzyme-linked immunospot (ELISPOT) method which enumerates individual TB-specific activated effector T cells.
Technical Note
Fast, simple, homogeneous assays for characterization of Fc receptor binding using AlphaLISA
In this technical note discover how the AlphaLISA human FcRn binding kit detects binding between FcRn (neonatal Fc receptor) and human IgG.
Technical Note
Plasmid DNA characterization in less than 2 minutes per sample
Rapidly and effectively separate the three primary isoforms of pDNA and provides precise purity assessment with the LabChip Plasmid DNA assay.
Technical Note
Rapid, Consistent, and Flexible Dispensing of AlphaLISA and HTRF Reagents Using the FlexDrop Plus Non-contact Dispenser
Download this Technical Note and discover the results obtained with the combination of FlexDrop iQ non-contact dispensing platform and AlphaScreen, AlphaLISA, and HTRF assay technologies.
Technical Note
Measure aggregated protein phosphorylation
This technical note shows that by combining the protein disaggregation kit with HTRF™ phospho immunoassays, you have a new, easy-to-use, and reliable approach for detecting all phosphorylation events during protein aggregation.
Technical Note
A simple method for preparing GPCR membrane model extracts from stable cell lines for use with the HTRF GTP Gi binding assay
This Technical Note provides a detailed method for preparing GPCR membrane model extracts from stable cell lines for use with the HTRF GTP Gi binding assay, including cell culture preparation, cell lysis, membrane extraction, and assay optimization.
Technical Note
Insider tips to optimize your GPCR signaling studies
This technical note provides valuable tips for performing Advanced phospho-ERK1/2 assays on adherent cell lines expressing GPCRs and will ensure optimal results.
Technical Note
Key success tips to perform β-arrestin 2 recruitment assays by using the B-arr2 recruitment kit with GPCRs
This technical note provides valuable tips for performing B-arr2 recruitment assays on adherent cell lines overexpressing GPCRs.
Technical Note
How to access true Kd values in Protein-Protein Interaction assays
This technical note draws on different case scenarios and assay formats to demonstrate the usefulness of the Cheng Prusoff equation in predicting PPI inhibitors potency.
Technical Note
Guidelines for optimizing protein-protein interaction assays using HTRF PPI reagents
This technical note reviews the general principles and steps in developing a protein-protein interaction assay using HTRF PPi reagents from Revvity assays.
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