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Application Note
DELFIA VICTOR Immunoassays- How to Optimize Rapid and Simple Immunoassays
In this application note, parameters affecting the performance characteristics of rapid and simple immunoassays have been optimized. These parameters were temperature, concentration of immunocomponents, dynamics (including diffusion), and the quality of immunocomponents.
Application Note
Developing novel tetraubiquitin substrates and AlphaLISA technology to provide next generation deubiquitinase assays
This application note presents novel biochemical DUB assays that use TUBEs to bind to tetraubiquitin substrates of at least three natural linkages. These assays, combined with Revvity’s AlphaLISA™ technology, allow for the measurement of protein interactions and large protein complexes in a no-wash, homogeneous assay.
Application Note
Detection of human matrix metalloproteinase 9 in complex sample matrices, a comparison of AlphaLISA and colorimetric ELISA immunoassays
This note compares two immunoassay technologies: ELISA (enzyme-linked immunosorbent assay) and AlphaLISA™ (amplified luminescent proximity-based homogeneous assay).
Application Note
HMGB1, IL-1β and IL-18 assays used together to discriminate pyroptosis and necroptosis
This application note shows that the release of HMGB1 induced in the context of pyroptosis and necroptosis can be easily monitored with the HTRF® HMGB1 kit.
Application Note
Download your application note on fibrosis characterization
This note describes deep investigations of the molecular mechanisms involved in tissue fibrosis, and occurring in the major effector cell types.
Application Note
Download your application note on nuclear receptor ligand identification with HTRF
This application note illustrates the use of HTRF PPI reagents to easily build robust and convenient biochemical NR/ CoA recruitment assays.
Application Note
Best practices for analyzing tumor xenografts
This technical note provides procedures and guidelines for performing HTRF phospho-protein assays on tumor xenografts, with a particular focus on the preparation of tumor lysates and working samples.
Application Note
Download your protocol on CD4 T cells differentiation into Th17 cells
In this application note is reported a protocol for the isolation of mouse naïve CD4+ T-cells and their differentiation into T-Reg and Th-17 cells.
Application Note
Download your application note on nuclear receptor ligand identification with HTRF
This application note illustrates the use of HTRF PPI reagents to easily build robust and convenient biochemical NR/CoA recruitment assays.
Application Note
A guideline for HTRF cell-based phospho-protein data normalization
This application note illustrates how to calculate a normalization value and interpret the results correctly when using htrf phosphospecific and total protein assays.
Application Note
HTRF probes all types of protein-protein interaction
HTRF probes all types of protein-protein interaction, from small to very large complexes
Application Note
Inhibition of FGFR signaling pathways studied in cancer cell lines using HTRF
In this application note, we show that a pan-FGFR inhibitor, AZD4547, used in different clinical phases depending on the cancer type, is able to inhibit or even suppress the abnormal phosphorylation of FGFRs and their key effectors in 3 different types of cancer cell lines: KG-1 (blood cancer), SNU-16 (gastric cancer), and KMS-11 (multiple myeloma).
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