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Technical Note
Guidelines for cell culture and lysis in different formats prior to HTRF detection
This Technical Note will provide you with guidelines on adapting the standard 96-well plate protocol for seeding and lysing cells to different culture formats such as T75 flasks, or 6-well, 12-well, and 24-well plates.
Technical Note
The AlphaLISA™ Approach to TNFR1 Measurement: Insights from Human Urine Samples
Detailed in this technical document is the method for assessing TNFR1 concentrations in human urine, showcasing a comparative analysis between samples from healthy individuals and those with type 2 diabetes.
Technical Note
The Advantages of AlphaLISA™ Technology: A Study with Human TNFR1
This technical note compares ELISA kits from two suppliers to the Human TNFR1 AlphaLISA™ Detection Kit to demonstrate the ease of use and benefits of Alpha technology.
Technical Note
AlphaLISA SET7/9 Histone H3-lysine N-methyltransferase assay.
this AlphaLISA immunoassay detects the mono-methylation of a biotinylated Histone H3 (1-21) peptide at lysine 4.
Technical Note
AlphaLISA SIRT1 p53 lysine 382 deacetylase assay
This AlphaLISA immunodetection assay measures the deacetylation of a biotinylated p53 (368-393) peptide acetylated at lysine 382.
Technical Note
Package insert for the T-SPOT.TB test
The T-SPOT.TB test is an in vitro diagnostic test for the detection of effector T cells that respond to stimulation by Mycobacterium tuberculosis antigens and is intended for use as an aid in the diagnosis of tuberculosis (TB) infection. The T-SPOT.TB test is a simplified enzyme-linked immunospot (ELISPOT) method which enumerates individual TB-specific activated effector T cells.
Technical Note
Fast, simple, homogeneous assays for characterization of Fc receptor binding using AlphaLISA
In this technical note discover how the AlphaLISA human FcRn binding kit detects binding between FcRn (neonatal Fc receptor) and human IgG.
Technical Note
AlphaLISA JMJD3 Histone H3-Lysine 27 demethylase assay
This AlphaLISA immunodetection assay measures the demethylation of a biotinylated Histone H3 (21-44) peptide tri-methylated at lysine 27.
Technical Note
Exposing and detecting membrane and intracellular proteins: AlphaLISA simplifies the detection of HO-1
This technical document reveals the application of AlphaLISA technology in measuring HO-1 concentrations within HeLa cell extracts.
Technical Note
Development of an AlphaLISA MEK1 kinase assay using full-length ERK2 substrate
This technical note illustrates one example of how these toolbox beads may be used to efficiently develop an assay for assessing MEK1 phosphorylation of a native ERK2 substrate.
Technical Note
AlphaLISA G9a Histone H3-Lysine N-methyltransferase assay
In this technical note discover how The AlphaLISA assay detects di-methylation of a biotinylated Histone H3 (1-21) peptide at lysine 9.
Technical Note
AlphaLISA EZH2 Histone H3-Lysine 27 N-methyltransferase assay
AlphaLISA assay detects dimethylation of a biotinylated Histone H3 (21-44) peptide at lysine 27. It uses anti-di/mono-methyl-Histone H3 Lysine 27 (H3K27me2-1) as the detection antibody
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