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Technical Note
Exposing and detecting membrane and intracellular proteins: AlphaLISA simplifies the detection of HO-1
This technical document reveals the application of AlphaLISA technology in measuring HO-1 concentrations within HeLa cell extracts.
Technical Note
Development of an AlphaLISA MEK1 kinase assay using full-length ERK2 substrate
This technical note illustrates one example of how these toolbox beads may be used to efficiently develop an assay for assessing MEK1 phosphorylation of a native ERK2 substrate.
Brochure
Guide
AlphaLISA assay development guide
A few simple steps are needed to develop an AlphaLISA™ analyte assay. This protocol is the starting point for the development of all AlphaLISA assays.
Guide
Discover alpha technology: illuminating protein interactions
Explore Alpha Technology: Illuminate protein interactions with no-wash assays. Discover large complexes and versatile targets.
Guide
User’s guide: Alpha protein:protein interaction assays
Explore AlphaScreen™ and AlphaLISA™ assays—where proximity meets luminescence. Discover protein interactions in a microplate format.
Technical Note
AlphaLISA G9a Histone H3-Lysine N-methyltransferase assay
In this technical note discover how The AlphaLISA assay detects di-methylation of a biotinylated Histone H3 (1-21) peptide at lysine 9.
Technical Note
AlphaLISA EZH2 Histone H3-Lysine 27 N-methyltransferase assay
AlphaLISA assay detects dimethylation of a biotinylated Histone H3 (21-44) peptide at lysine 27. It uses anti-di/mono-methyl-Histone H3 Lysine 27 (H3K27me2-1) as the detection antibody
Technical Note
AlphaLISA SIRT1 Histone H3-Lysine 4 deacetylase assay
This AlphaLISA immunodetection assay measures the deacetylation of a biotinylated Histone H3 (1-21) peptide acetylated at lysine 4.
Technical Note
AlphaLISA KAT5 (TIP60) assay
This AlphaLISA™ immunodetection assay measures the acetylation of a Histone H4 (1-25) peptide at the N-terminal lysine residues.
Flyer
The newest AlphaLISA and HTRF kits
Discover Revvity's AlphaLISA™ and HTRF™ assays: ready-to-use, no-wash kits offering improved sensitivity and dynamic range for your targets.
Technical Note
AlphaLISA in urine: Detecting kidney injury marker KIM-1
This document highlights the capability of AlphaLISA technology to identify the kidney injury biomarker KIM-1 in urine samples, achieving detection levels as low as 14 pg/mL.
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