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Revvity’s luminescence assays include reporter gene and ATP-monitoring and allow high sensitivity in a convenient microplate format.
Application Note
Quantify low abundant mIL-17A homodimer in mouse serum and plasma
This application note demonstrates the quantification of low levels of mIL-17A homodimer in mouse serum and plasma samples using the AlphaLISA mIL‑17A homodimer detection kit.
Application Note
Development of AlphaLISA technology assays for the detection of neutralizing antibodies in human serum
We report here the development of two AlphaLISA functional assays, a cell-based and a biochemical assay, for the detection of neutralizing ADA in human serum samples.
Technical Note
Development of an AlphaLISA assay to measure a DNA-protein interaction with a strong affinity
In this technical note, we show how to easily develop a robust AlphaLISA™ assay that measures a tight binding interaction between a protein and a DNA oligonucleotide.
Technical Note
Multiplex assays with Alpha SureFire Ultra Multiplex technology using the EnVision multimode plate reader
In this technical note, we will describe how to set up Alpha SureFire® Ultra™ Multiplex kits on the EnVision™ multimode plate reader.
Whitepaper
Understanding the ‘how’ and ‘why’ of spectral unmixing
Understanding the “why” and “how” of using spectral unmixing in your in vivo imaging studies
Technical Note
Development of an AlphaLISA assay to measure and screen inhibitors of the p53-MDM2 interaction
Development of an AlphaLISA Assay to Measure and Screen Inhibitors of the p53-MDM2 Interaction
Literature - Publication Review
Clinically translatable cytokine delivery platform for eradication of intraperitoneal tumors
Researchers use a clinically translatable cytokine delivery platform in an ovarian mouse model validated using IVIS® optical imaging and IVISbrite™ D-luciferin bioluminescent substrate.
Application Note
LSC Sample Preparation and Counting of Biological Samples
In general, all biological samples contain components not generally found in any other sample types, therefore it is of benefit to understand the composition of these samples to fully understand the need for the slightly unusual sample preparation methods.
Technical Note
PROTAC degraders targeting CDK4/CDK6 for the study of cell cycle regulation in oncology
Explore the cell cycle with AlphaLISA™ SureFire® Ultra™ and investigate Cyclin D1 and key proteins' roles in cancer prognosis, PROTAC, and NRG1 treatments.
Application Note
Evaluation of drug selectivity and efficacy using PROTACs in a 3D co-culture spheroid model
The aim of this study was to evaluate the efficacy and selectivity of SJF1528, a pan-EGFR PROTAC, and MS39, a mutant-specific EGFR L858R PROTAC, while exploring the use of both high-content imaging and no-wash HTRF immunoassays in encapsulated 3D spheroid models.
Literature - Publication Review
Deciphering the properties of neuronal nicotinic acetylcholine receptors using radiolabeled α-Bungarotoxin
Deciphering the Properties of Nicotinic Acetylcholine Receptors Using 125I-α-Bungarotoxin
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